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Wow, Mother Brain is a beast. Does she have the most HP of any boss in any 2D metroid? Id think Metroid Queen has a lot but probably not that much. I guess youd have to compare Mother Brain's 2nd form and discard the 1st & 3rd when comparing to others since they are special circumstances.
Shifty Leader
That M2k2 guy
MB second form takes 30 more regular missiles than MQ does (even though she's killed by like 4 bombs in the stomach), but MB2 doesn't seem so bad because you have super missiles. Also, the strongest beam combination is 9x as strong as missiles in SM. I completely forget SA-X's and OM's health, but the beams in Fusion are so hella strong that it doesn't even matter.

These are all off the top of my head, though, but I think it's right.
I like Big Butts and I can not lie
Ridley in Super Metroid also has 18000 health (charged ice-wave-plasma does 900), Ridley in Zero Mission has 1000 health (super missile does 100), Ridley in fusion has 4500 health (charged wave with flare does ~420)
Shifty Leader
That M2k2 guy
You mean just the first frame, or is that the maximum amount of damage you can do in one shot?
I like Big Butts and I can not lie
Maximum you'll get with a horizontal shot